Prevalence of hemoglobin variants in a diabetic population at high risk of hemoglobinopathies and optimization of HbA1c monitoring by incorporating HPLC in the laboratory workup

Libyan journal of medicine

Kahena Bouzid¹ 

关键词: hemoglobinopathies;    HbA1c;    HPLC;    diabetes mellitus;    prevalence;    Tunisia;   

DOI  :  10.3402/ljm.v9.25768

学科分类：医学（综合）

来源: Co-Action Publishing

【 摘 要 】

BackgroundIn Tunisia, diabetes mellitus and hemoglobinopathies are major public health problems. Glycated hemoglobin (HbA1c) is recommended for long-term monitoring of diabetes mellitus, but the presence of hemoglobin variants may interfere with HbA1c measurement. The aim was to determine the prevalence of hemoglobin variants in Tunisian diabetics and optimize the monitoring of diabetics using HbA1c.MethodsThe study enrolled 9,792 Tunisian diabetic patients. HbA1c was measured by cation-exchange high-pressure liquid chromatography (HPLC). All the chromatograms were analyzed for the presence of Hb variants.ResultsWe identified 228 cases (2.33%) of Hb variants with D-10 HPLC (Bio-Rad) 191 with HbA/S trait, 27 with HbA/C trait, and 10 hemoglobin variants with the mention ‘Variant-Window’ on the chromatograms and subsequently identified as HbA/S on Variant I HPLC (Bio-Rad). Thus, the prevalence of HbS was 2.05%. We did not find any homozygous variant. All HbA1c results were reported to the treating physician...

【 授权许可】

CC BY