American Journal of Cancer Research | |
MicroRNA-143 acts as a tumor suppressor by targeting hexokinase 2 in human prostate cancer | |
Peng Zhou1  | |
关键词: miR-143; prostate cancer; growth; hexokinase 2; | |
DOI : | |
学科分类:肿瘤学 | |
来源: e-Century Publishing Corporation | |
【 摘 要 】
MicroRNAs (miRNAs) are small non-coding RNAs that play important roles in cancer progression through regulating gene expression. Down-regulation of miR-143 has been reported in a number of cancers. However, the biological functions of miR-143 in prostate cancer remain largely unexplored. In this study, we showed that miR-143 expression was reduced in approximately 62.5% of the specimens examined. By loss-of-function and gain-of-function studies in human prostate cancer PC-3 cells, we demonstrated that miR-143 has an inhibitory effect on cell proliferation as evidenced by decreased cell viability, increased cell apoptosis and cell cycle arrest at the G1/S transition. Furthermore, we identified hexokinase 2 (HK2), a metabolic enzyme that executes the first step of aerobic glycolysis, as a target of miR-143 in prostate cancer. Knockdown of HK2 recapitulated the effects of miR-143 and accompanied with decreased glucose metabolism. Taken together, these data indicate that miR-143/HK2 axis plays an important role in the development of prostate cancer and represents a potential therapeutic target for prostate cancer.
【 授权许可】
CC BY-NC
【 预 览 】
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RO201904033229574ZK.pdf | 1134KB | download |