L-arginine protects against ethylene glycol-induced gastric mucosal damage in rats: immunohistochemical and electron microscopic study

Turkish Journal of Biology

SAMAH KANDEEL¹ 

关键词: Ethylene glycol;    L-arginine;    stomach;    caspase-3;    immunohistochemistry;    ultrastructure;    rat;   

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

Ethylene glycol is an alcohol widely used in industry as an antifreeze. It affects the normal gastric mucosal defense mechanisms, inducing surface ulcerations. L-arginine is a precursor of nitric oxide that exhibits multiple biological properties; among them is the acceleration of wound healing, especially in the stomach. Caspase-3 immunohistochemistry, and semithin and ultrathin section examinations were used to investigate the possible protective effect of L-arginine on ethylene glycol-induced gastric mucosal damage in rats. Three animal groups were used: group A (control group), group B (received 3.3 mL/kg ethylene glycol for 2 weeks), and group C (received 200 mg/kg of L-arginine and 3.3 mL/kg ethylene glycol for 2 weeks). By examination, group B showed significant increases in caspase-3 positive cells in comparison to the control, semithin sections revealed surface disruption and ulcerations, and ultrathin sections showed multiple pathological changes in cells lining the gastric mucosal glands. In group C significant decreases in caspase-3 positive cells were encountered and the other pathological findings in group B were found to be reversed. Therefore, we conclude that ethylene glycol-induced damage to the gastric mucosa could be prevented by L-arginine administration.

【 授权许可】

Unknown