Fluorescent Bead–DNA Conjugate‐based Dual Signal Amplification Technology

Bulletin of the Korean Chemical Society

Hae Ryong Lee²  Taisun Kim²  Keum‐²  Sang Cheol Kim³  Satish Balasaheb Nimse⁴  Mukesh Digambar Sonawane⁴  Soo Song⁴ 

[1] 161 Korea;702 Korea;Biometrix Technology, Inc. Chuncheon 200‐Institute for Applied Chemistry and Department of Chemistry Hallym University Chuncheon 200‐

关键词: Microarray;    Fluosphere;    Fluoro‐;    microbeads;    Signal amplification;    ;    9G DNA chips;   

DOI  :  10.1002/bkcs.10744

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

One of the major challenges in DNA microarrays for their widespread application is assay sensitivity. Therefore, to achieve higher sensitivity, we developed a dual signal amplification technology based on the fluorescence beads (FB). It is the first report of the development of a dual signal amplification technology for DNA microarrays by synthesizing and applying the FB–DNA conjugates. The FB–DNA conjugates and Cy5‐T1‐FB‐mixed‐DNA demonstrated ~250–325‐fold increase in sensitivity when compared with the conventional use of Cy5‐labeled oligonucleotides such as Cy5‐T1. Furthermore, the Cy5‐T1‐FB‐mixed‐DNA demonstrated ~5–1.3‐fold increase in the sensitivity when compared with that of the FB–DNA conjugates alone.

【 授权许可】

Unknown