Detection of Circulating hcmv-miR-UL112-3p in Patients with Glioblastoma, Rheumatoid Arthritis, Diabetes Mellitus and Healthy Controls

PLoS One

Anders Hamsten¹  Linda Mellbin²  Lars Rydén²  Weng-Onn Lui³  Afsar Rahbar⁴  Koon-Chu Yaiw⁴  Belghis Davoudi⁴  Abdul-Aleem Mohammad⁴  Cecilia Söderberg-Nauclér⁴  Giuseppe Stragliotto⁴  Anca Catrina⁵ 

[1] Atherosclerosis Research Unit, Center for Molecular Medicine, Karolinska Institute, Stockholm, Sweden;Cardiology Unit, Department of Medicine Solna, Karolinska Institute, Stockholm, Sweden;Department of Oncology-Pathology, Karolinksa Institutet, Cancer Center Karolinska, Karolinska University Hospital, Stockholm, Sweden;Experimental Cardiovascular Research Unit, Department of Medicine-Solna, Center for Molecular Medicine, Karolinska Institute, Stockholm, Sweden;Rheumatology Unit, Department of Medicine, Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden

关键词: MicroRNAs;    Human cytomegalovirus;    Glioblastoma multiforme;    Rheumatoid arthritis;    Cytomegalovirus infection;    Viral persistence and latency;    Diabetes mellitus;    Enzyme-linked immunoassays;   

DOI  :  10.1371/journal.pone.0113740

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Background microRNAs (miRNA) are 18–22 nucleotides long non-coding RNAs that regulate gene expression at a post-transcriptional level. Human cytomegalovirus (HCMV) encodes at least 26 known mature miRNAs. hcmv-miR-UL112-3p (miR-UL112-3p) is the most well characterized HCMV miRNA, which is suggested to play role in establishment and maintenance of viral latency. Elevated miR-UL112-3p levels have been reported to be present in plasma of patients with hypertension.Objectives In this study, we aimed to quantify miR-UL112-3p levels in the plasma/serum of patients with Diabetes Mellitus (DM; from the DIGAMI-2 cohort), Glioblastoma multiforme (GBM), Rheumatoid Arthritis (RA) and Healthy Controls (HC).Study Design Total RNA was isolated from plasma/serum samples of 87 patients and controls, a TaqMan miRNA assay was performed to detect miR-UL112-3p and the copy numbers were normalized to 10 ng of total RNA. HCMV IgG and IgM were analysed using ELISA.Results HCMV miR-UL112-3p was detected in 14/27 (52%) of DM, 5/20 (25%) of GBM, 1/20 (5%) of RA patients and in 2/20 (10%) of HC, respectively. Anti-HCMV IgG was detected in 85%, 65%, 75% of patients and 70% of HC, respectively. Anti-HCMV IgM was found only in one GBM patient of 87 examined patients and controls.Conclusions A higher prevalence of miR-UL112-3p was detected in DM and GBM patients than in RA patients and HC. Elevated levels of miR-UL112-3p and higher prevalence of HCMV IgG were observed in DM patients. Whether the presence of circulating miR-UL112-3p denotes a biomarker of HCMV latency or active replication in patients warrants further investigation.

【 授权许可】

CC BY