期刊论文详细信息
PLoS One
Identification of Short Hairpin RNA Targeting Foot-And-Mouth Disease Virus with Transgenic Bovine Fetal Epithelium Cells
Guangpeng Li1  Lei Cheng1  Chengqiang He2  Jianbin Li3  Jianming Wu3  Xiao Liu3  Hongjun Yang3  Hongmei Wang3  Hongbin He3  Jifeng Zhong3  Wenhao Liu3  Li Yu4  Yunping Dai5  Fangrong Ding5 
[1] College of Life Science, Inner Mongolia University, Huhehaote, People's Republic of China;College of Life Science, Shandong Normal University, Jinan, People's Republic of China;Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, People's Republic of China;Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Harbin, People's Republic of China;State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, People's Republic of China
关键词: Viral replication;    Tongue;    Fetuses;    Viral genes;    Foot and mouth disease;    Epithelium;    RNA interference;    Genetically modified animals;   
DOI  :  10.1371/journal.pone.0042356
学科分类:医学(综合)
来源: Public Library of Science
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【 摘 要 】

Background Although it is known that RNA interference (RNAi) targeting viral genes protects experimental animals, such as mice, from the challenge of Foot-and-mouth disease virus (FMDV), it has not been previously investigated whether shRNAs targeting FMDV in transgenic dairy cattle or primary transgenic bovine epithelium cells will confer resistance against FMDV challenge. Principal Finding Here we constructed three recombinant lentiviral vectors containing shRNA against VP2 (RNAi-VP2), VP3 (RNAi-VP3), or VP4 (RNAi-VP4) of FMDV, and found that all of them strongly suppressed the transient expression of a FLAG-tagged viral gene fusion protein in 293T cells. In BHK-21 cells, RNAi-VP4 was found to be more potent in inhibition of viral replication than the others with over 98% inhibition of viral replication. Therefore, recombinant lentiviral vector RNAi-VP4 was transfected into bovine fetal fibroblast cells to generate transgenic nuclear donor cells. With subsequent somatic cell cloning, we generated forty transgenic blastocysts, and then transferred them to 20 synchronized recipient cows. Three transgenic bovine fetuses were obtained after pregnant period of 4 months, and integration into chromosome in cloned fetuses was confirmed by Southern hybridization. The primary tongue epithelium cells of transgenic fetuses were isolated and inoculated with 100 TCID50 of FMDV, and it was observed that shRNA significantly suppressed viral RNA synthesis and inhibited over 91% of viral replication after inoculation of FMDV for 48 h. Conclusion RNAi-VP4 targeting viral VP4 gene appears to prevent primary epithelium cells of transgenic bovine fetus from FMDV infection, and it could be a candidate shRNA used for cultivation of transgenic cattle against FMDV.

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