Optimization of Tenocyte Lineage-Related Factors from Mesenchymal Stem Cells:

Foot & Ankle Orthopaedics

Seung YeolLee¹ 

关键词: tenocyte;    tenogenic differentiation;    tonsil-derived mesenchymal stem cell;    bone marrow-derived mesenchymal stem cell;    TGF-b;    response surface methodology;   

DOI  :  10.1177/2473011418S00310

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

Category:Basic Sciences/BiologicsIntroduction/Purpose:Researchers should consider various potential factors that affect tenogenic differentiation of MSCs. Numerous experimental settings are associated with high cost and time. Response surface methodology (RSM), a component in the design of experiments (DOE), is gaining recognition as a powerful tool in optimizing conditions for the production of industrially important products such as chemicals and enzymes. The purpose of this study was to access the differential effects of transforming growth factor beta 3 (TGF-β3) on the tenogenesis of tonsil-derived mesenchymal stem cells (T-MSCs) and bone marrow-derived mesenchymal stem cells (BM-MSCs) using RSM.Methods:Bone marrow was collected from four patients (mean age: 79.0±2.2) and mononuclear cells were separated. The tonsillar tissues were collected from four patients (mean age: 7.6±0.6). After isolation of MSCs, they were treated with 5ng/ml and 10ng/ml of TGF-β3 with vehicle control. The full-factorial expe...

【 授权许可】

CC BY