Tenogenic differentiation of mesenchymal stem cells:

Foot & Ankle Orthopaedics

Seung YeolLee¹ 

关键词: tenocyte;    tenogenic differentiation;    tonsil-derived mesenchymal stem cell;    bone marrow-derived mesenchymal stem cell;    adipose tissue-derived mesenchymal stem cell;    TGF-b;   

DOI  :  10.1177/2473011418S00309

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

Category:Basic Sciences/BiologicsIntroduction/Purpose:Tendon repair has been a challenging issue for surgeons in treating. Although tissue engineering with mesenchymal stem cells (MSC) have been used for tendon repair in both in vivo and in vitro, the stem cells are obtained through invasive procedures, and there is usually a lack of adequate numbers for clinical use. The purpose of this study was to compare the potential of tri-lineage differentiation and to investigate the potential of tenogenic differentiation of human tonsil derived MSCs (T-MSCs), bone marrow derived MSCs (BM-MSCs), and adipose tissue derived MSCs (AD-MSCs).Methods:Each tissue was obtained from 8 patients. After isolation of MSCs, flow cytometry analysis was used to characterize the phenotypes of the MSCs. Differentiation capacity to adipo-, osteo-, and chondrocytes were induced by culturing each MSCs for 3 weeks in commercially available media. Each MSCs was treated with 5ng/ml and 10ng/ml of TGF-ß3 with vehicle control.Results:Immun...

【 授权许可】

CC BY