Rapid Cellular Turnover in Adipose Tissue

PLoS One

Alessandra Rigamonti¹  Chad A. Cowan¹  Kristen Brennand²  Frank Lau³ 

[1] Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Boston, Massachusetts, United States of America;Doctorate of Prenatal Science, Fetal Diagnosis and Therapy, University of Milan, Milan, Italy;Salk Institute for Biological Studies, La Jolla, California, United States of America

关键词: Adipocytes;    Adipose tissue;    Obesity;    Bromodeoxyuridine labeling;    Fats;    DAPI staining;    Nuclear staining;    Cell cycle and cell division;   

DOI  :  10.1371/journal.pone.0017637

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

It was recently shown that cellular turnover occurs within the human adipocyte population. Through three independent experimental approaches — dilution of an inducible histone 2B-green fluorescent protein (H2BGFP), labeling with the cell cycle marker Ki67 and incorporation of BrdU — we characterized the degree of cellular turnover in murine adipose tissue. We observed rapid turnover of the adipocyte population, finding that 4.8% of preadipocytes are replicating at any time and that between 1–5% of adipocytes are replaced each day. In light of these findings, we suggest that adipose tissue turnover represents a possible new avenue of therapeutic intervention against obesity.

【 授权许可】

CC BY