Reduction of Microorganisms via Green Tea Bioreactor Treatment and Rapid Microbial Determination with Quantum Dots Labeled Method

Biochemistry and Biophysics

Yajuan Su¹ 

关键词: Quantum Dots;    Green Tea Powder;    Bioreactor;    Microorganisms’ Reduction;   

DOI  :  

学科分类：生物化学/生物物理

来源: Science and Engineering Publishing Company

【 摘 要 】

Abstract: Cadmium–telluride (CdTe) quantum dots as a fluorescence label, and a rapid, simple, and sensitive quantum dots (QDs) labeled method was employed to detect the total count of bacteria and fungi, using 1-Ethyl-3-(3-dimethyllaminopro- pyl)-carbodiimide (EDC) as a crosslinker. CdTe quantum dots could successfully be conjugated with the representive bacteria Escherichia coli, mold Aspergillus niger and yeast Saccharomyces cerevisiae. This method could count bacteria and fungi with content of 102 CFU/mL. The determination of total count of bacteria and fungi with fluorescence method for three samples of tap water, river water and lake water were similar to the standard plate count method. The green tea powder bioreactor was constructed to treat the three samples, and the QDs labeled method was used to test the reduced bacteria and fungi, and the results revealed that this green tea powder bioreactor could effectively reduce the amount of microorganisms of the three samples.

【 授权许可】

Unknown