PLoS One | |
Alcelaphine Herpesvirus-1 (Malignant Catarrhal Fever Virus) in Wildebeest Placenta: Genetic Variation of ORF50 and A9.5 Alleles | |
Felix Lankester1  Dawn M. Grant2  Ahab Ndabigaye3  David M. Haig3  George C. Russell4  Sarah Cleaveland4  Ahmed Lugelo5  Nicholas Mnyambwa6  Rudovick Kazwala6  Julius Keyyu6  Valerie Relf7  | |
[1] Boyd Orr Centre for Population and Ecosystem Health, Institute of Biodiversity, Animal Health & Comparative Medicine, University of Glasgow, Glasgow, G12 8QQ, United Kingdom;Department of Science and Laboratory Technology, Dar es Salaam Institute of Technology, Dar es Salaam, Tanzania;Faculty of Veterinary Medicine, Sokoine University of Agriculture, Morogoro, Tanzania;Moredun Research Institute, Pentlands Science Park, Penicuik, Edinburgh, United Kingdom;Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, 99164, United States of America;School of Life Sciences and Bioengineering, Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania;Tanzania Wildlife Research Institute, Arusha, Tanzania | |
关键词: Wildebeest; Polymerase chain reaction; Sequence alignment; DNA sequence analysis; Cattle; Phylogenetic analysis; Nucleotide sequencing; Sequence analysis; | |
DOI : 10.1371/journal.pone.0124121 | |
学科分类:医学(综合) | |
来源: Public Library of Science | |
【 摘 要 】
Alcelaphine herpesvirus–1 (AlHV-1), a causative agent of malignant catarrhal fever in cattle, was detected in wildebeest (Connochaetes taurinus) placenta tissue for the first time. Although viral load was low, the finding of viral DNA in over 50% of 94 samples tested lends support to the possibility that placental tissue could play a role in disease transmission and that wildebeest calves are infected in utero. Two viral loci were sequenced to examine variation among virus samples obtained from wildebeest and cattle: the ORF50 gene, encoding the lytic cycle transactivator protein, and the A9.5 gene, encoding a novel polymorphic viral glycoprotein. ORF50 was well conserved with six newly discovered alleles differing at only one or two base positions. In contrast, while only three new A9.5 alleles were discovered, these differed by up to 13% at the nucleotide level and up to 20% at the amino acid level. Structural homology searching performed with the additional A9.5 sequences determined in this study adds power to recent analysis identifying the four-helix bundle cytokine interleukin-4 (IL4) as the major homologue. The majority of MCF virus samples obtained from Tanzanian cattle and wildebeest encoded A9.5 polypeptides identical to the previously characterized A9.5 allele present in the laboratory maintained AlHV-1 C500 strain. This supports the view that AlHV-1 C500 is suitable for the development of a vaccine for wildebeest-associated MCF.
【 授权许可】
CC BY
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