Genome-wide association study for grain yield and related traits in elite wheat varieties and advanced lines using SNP markers

PLoS One

Jian-Bang Hu¹  Hui Shao¹  Hai-Ping Zhang¹  Gen-Lou Sun¹  Yu-Lei Zhu¹  Jie Lu¹  Cheng Chang²  Peng Liu²  Heng Zhang²  Chuan-Xi Ma²  Sheng-Xing Wang²  Xian-Chun Xia²  De-Xin Zhang² 

[1] Key Laboratory of Wheat Biology and Genetic Improvement in the Southern Yellow & Huai River Valley, Ministry of Agriculture, Hefei, Anhui, China;College of Agronomy, Anhui Agricultural University;Department of Biology, Saint Mary’s University, Halifax, Nova Scotia, Canada;Institute of Crop Science, National Wheat Improvement Centre/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China

关键词: Wheat;    Gene mapping;    Molecular genetics;    Genome-wide association studies;    Phenotypes;    Quantitative trait loci;    Genetic loci;    Population genetics;   

DOI  :  10.1371/journal.pone.0188662

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Genetic improvement of grain yield is always an important objective in wheat breeding. Here, a genome-wide association study was conducted to parse the complex genetic composition of yield-related traits of 105 elite wheat varieties (lines) using the Wheat 90K Illumina iSelect SNP array. Nine yield-related traits, including maximum number of shoots per square meter (MSN), effective number of spikes per square meter (ESN), percentage of effective spike (PES), number of kernels per spike (KPS), thousand-kernel weight (TKW), the ratio of kernel length/kernel width (RLW), leaf-area index (LAI), plant height (PH), and grain yield (GY), were evaluated across four environments. Twenty four highly significant marker-trait associations (MTAs) (P < 0.001) were identified for nine yield-related traits on chromosomes 1A, 1D, 2A (2), 3B, 4A (2), 4B, 5A (4), 5B (4), 5D, 6B (2), 7A (2), and 7B (3), explaining 10.86–20.27% of the phenotypic variations. Of these, four major loci were identified in more than three environments, including one locus for RLW (6B), one locus for TKW (7A), and two loci for PH (7B). A cleaved amplified polymorphic sequence (CAPS) marker Td99211 for TKW on chromosome 5A was developed and validated in both a natural population composed of 372 wheat varieties (lines) and a RIL population derived from the cross of Yangxiaomai × Zhongyou 9507. The CAPS marker developed can be directly used for marker-assisted selection in wheat breeding, and the major MTAs identified can provide useful information for fine-mapping of the target genes in future studies.

【 授权许可】

CC BY