Pathogenicity of indigenous soil isolate of Bacillus thuringiensis to Helicoverpa armigera Hübner 1809 (Lepidoptera: Noctuidae)

Egyptian Journal of Biological Pest Control

Chun-Sen Ma¹  Muneeba Arif Khan¹  Salman Ahmad²  Muhammad Zeeshan Majeed³  Muhammad Asam Riaz⁴ 

[1]Department of Entomology, College of Agriculture, University of Sargodha, Sargodha, Pakistan

[2]Department of Entomology, University of Georgia, Athens, USA

[3]State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’

[4]s Republic of China

关键词: Bacillus thuringiensis;    Microbial insecticides;    Helicoverpa armigera;    Toxicity evaluation;   

DOI  :  10.1186/s41938-018-0041-4

学科分类：动物科学

来源: Springer

【 摘 要 】

This study evaluated the pathogenicity of indigenous soil isolate of Bacillus thuringiensis (Bt) strains, applied without and along with 1.0% MgCl2 salt, to Helicoverpa armigera Hübner (Lepidoptera: Noctuidae). Toxicity and effect of Bt isolate on larval development (weight) were assessed using in vitro bioassays. Six concentrations of the tested Bt with salt (i.e., 1.0 × 107, 0.5 ×  105, 1.0 × 105, 1.5 × 105, and 2.0 × 105 cfu/ml), five without salt (i.e., 0.5 × 105, 1.0 × 105, 1.5 × 105, and 2.0 × 105 cfu/ml), and control were bioassayed against third-instar larvae of H. armigera under a complete randomized design (CRD), with four replications. Results revealed that both larval mortality and weight changes were significantly affected by time (F5, 19 = 35.98; P <  0.001 and F5, 19 = 11.01; P < 0.001, respectively) and treatments (F5, 19 = 27.45; P < 0.001 and F5, 19 = 25.07; P < 0.001). The highest larval mortality (91.1%) was exhibited by the highest concentration (1.0 × 107 cfu/ml), followed by 2.0 × 105 cfu/ml concentration, without salt (88.9%) and with salt (66.7%). Median lethal concentration (LC50) values of isolated Bt strain were 1.7 and 1.27 × 105 cfu/ml, without salt, and 1.80 and 1.13 × 105 cfu/ml, with salt, at 96 and 120 h, respectively. Regarding the impact of Bt isolate on larval development, treatment with the highest concentration (1.0 × 107 cfu/ml) had the most significant and negative impact on larval weight change (R2 = 0.53), followed by 2.0 × 105 cfu/ml Bt concentration (R2 = 0.40). There was no obvious synergistic or additive effect, but rather an inhibition was observed on the pathogenicity potential or larvicidal effect of Bt isolate.

【 授权许可】

CC BY