Gene expression profiles of RAW264.7 macrophages stimulated with preparations of LPS differing in isolation and purity:

Innate Immunity

Holly RRutledge¹ 

关键词: Lipopolysaccharide;    endotoxin;    LPS preparation;    macrophage;    gene expression;    microarray;   

DOI  :  10.1177/1753425910393540

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Lipopolysaccharide is a major component of the cell wall of Gram-negative bacteria and a potent stimulator of innate immune response via TLR4. Studies on the LPS action both in vivo and in vitro have used different preparations of LPS, including ultra-pure LPS (LIST) and a less pure but less expensive form (Sigma) isolated from Escherichia coli serotype O111:B4. The difference between the effects of these compounds has not been well studied although this information is important in understanding TLR stimulation. In this study, we compared response of RAW264.7 macrophage cells treated LIST or Sigma LPS for 6 h and 24 h. Gene expression data were analyzed to identify specific genes and pathways that are in common and unique to the two LPS preparations. Seven hundred fifty-five genes were differentially expressed at 6 h in response to Sigma LPS and 973 were differentially expressed following LIST LPS treatment, with 503 in common. At 24 h, Sigma LPS induced or repressed 901 genes while 1646 genes were differ...

【 授权许可】

CC BY