American Journal of Translational Research | |
MiR-146a activates WAVE2 expression and enhances phagocytosis in lipopolysaccharide-stimulated RAW264.7 macrophages | |
Zhongwei Cao1  | |
关键词: MiR-146a; endotoxin; WAVE2; phagocytosis; macrophage; | |
DOI : | |
学科分类:医学(综合) | |
来源: e-Century Publishing Corporation | |
【 摘 要 】
MiR-146a has been shown to play a critical role in cell immunity and phagocytosis, processes that require rearrangement of the cytoskeleton. However, the detailed mechanism by which miR-146a regulates these events remains elusive. Here, we used luciferase reporter and protein assays to show that the cytoskeleton-regulatingprotein verprolin-homologous protein 2 (WAVE2), is a direct target of miR-146a. MiR-146a overexpression resulted in a decrease in WAVE2 protein expression under endotoxin-free culture conditions. Unexpectedly, however, miR-146a activated rather than repressed the expression of WAVE2 in macrophage RAW264.7 cells when cultured continuously in the presence of endotoxin. Furthermore, we demonstrated that miR-146a induced WAVE2 expression and enhanced phagocytosis in lipopolysaccharide-stimulated RAW264.7 macrophages. Our study suggests that lipopolysaccharide- induced miR146a indirectly activates WAVE2 expression; thus, facilitating cytoskeletal reorganization and phagocytosis in lipopolysaccharide-stimulated macrophages.
【 授权许可】
CC BY-NC
【 预 览 】
Files | Size | Format | View |
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RO201901231394074ZK.pdf | 797KB | download |