Replication or death: distinct fates of pathogenic Leptospira strain Lai within macrophages of human or mouse origin:

Innate Immunity

Shijun Li¹ 

关键词: Apoptosis;    intracellular fate;    Leptospira interrogans;    lysosome;    macrophages;    phagocytosis;   

DOI  :  10.1177/1753425909105580

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Pathogenic leptospires evoke severe diseases in humans but only cause mild chronic or asymptomatic infection in many host animals. The reasons for this diversity of infection remain unclear. Here, we demonstrated that Leptospira interrogans serovar Lai strain Lai had a similar ability to adhere to and enter primary and immortal (THP-1 and J774A.1) macrophages from human and mouse, but its intracellular fate in human macrophages differed markedly from that in mouse. The leptospires resided within membrane-bound vacuoles in the murine macrophages, but occurred free in the cytosol of human macrophages, with no surrounding vesicular membrane. Most leptospires in murine macrophages co-localized with the late-endosomal/lysosomal marker LAMP-1 and then were killed by lysosomal hydrolases, while most leptospires in human macrophages did not co-localize with this marker and survived. Enumeration of colony-forming units plus quantitative fluorimetry showed that in human, but not in murine, macrophages, the amounts ...

【 授权许可】

CC BY