Innate responses to small interfering RNA pools inhibiting herpes simplex virus infection in astrocytoid and epithelial cells:

Innate Immunity

HenrikPaavilainen¹ 

关键词: Antiviral;    herpes simplex virus;    interferon;    RNA interference;    small interfering RNA;   

DOI  :  10.1177/1753425914537921

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Herpes simplex virus (HSV) is a human pathogen that can cause severe diseases such as encephalitis, keratitis and neonatal herpes. Control of HSV infection may be achieved by using small interfering (si)RNAs. We have designed and enzymatically produced pools of siRNAs targeting HSV. In addition to the target-specific effects, such siRNAs may induce innate immunity responses that may contribute to antiviral effects. HSV has versatile ways of modulating innate immunity, and it remains unclear whether HSV-specific antiviral treatment would benefit from the potential immunostimulatory effects of siRNAs. To address this, cell lines derived from epithelium and nervous system were studied for innate immunity reactions to HSV infection, to siRNA treatment, and to a combination of treatment and infection. In addition, the outcome of HSV infection was quantitated. We show that innate immunity reactions vary drastically between the cell lines. Moreover, our findings indicate only a minimal relation between the antiv...

【 授权许可】

CC BY