In Vivo Function and Evolution of the Eutherian-Specific Pluripotency Marker UTF1

PLoS One

Akihiko Okuda¹  Toshiyuki Yamagishi²  Hidemasa Kato³  Satoru Takahashi³  Masatsugu Ema⁴  Fumitoshi Ishino⁴  Yoko Satta⁵  Yukako Katsura⁵  Masataka Hirasaki⁶  Masayoshi Kamon⁶  Tomoaki Hishida⁶  Ayumu Suzuki⁶  Miyuki Katano⁶  Jennifer A. Marshall Graves⁷  Janine E. Deakin⁷  Yo-ichi Nabeshima⁸  Yoko Nabeshima⁸  Yoko Kuroki⁹  Ryuichi Ono^1,10  Masazumi Nishimoto^1,11 

[1] Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Kawaguchi, Saitama, Japan;Department of Anatomy and Cell Biology, Graduate School of Medicine, Osaka City University, Osaka, Japan;Department of Anatomy and Embryology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Japan;Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University, 2-3-10 Kandasurugadai, Chiyoda-ku, Tokyo, Japan;Department of Evolutionary Studies of Biosystems, The Graduate University for Advanced Studies (Sokendai), Hayama, Kanagawa, Japan;Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, Yamane Hidaka, Saitama, Japan;Evolution, Ecology, and Genetics, Research School of Biology, The Australian National University, Canberra, Australian Capital Territory, Australia;Foundation for Biomedical Research and Innovation, 1-5-4 Minatojima-minamimachi, Chuo-ku, Kobe, Japan;La Trobe Institute of Molecular Science, La Trobe University, Melbourne, Victoria, Australia;Laboratory for Immunogenomics, RIKEN Research Center for Allergy and Immunology, Tsurumi-ku, Yokohama, Kanagawa, Japan;Radioisotope Experimental Laboratory, Research Center for Genomic Medicine, Saitama Medical University, Yamane Hidaka, Saitama, Japan

关键词: Embryos;    Mammalian genomics;    Pluripotency;    DNA sequence analysis;    Blastocysts;    Bird genomics;    Gene targeting;    Sequence databases;   

DOI  :  10.1371/journal.pone.0068119

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Embryogenesis in placental mammals is sustained by exquisite interplay between the embryo proper and placenta. UTF1 is a developmentally regulated gene expressed in both cell lineages. Here, we analyzed the consequence of loss of the UTF1 gene during mouse development. We found that homozygous UTF1 mutant newborn mice were significantly smaller than wild-type or heterozygous mutant mice, suggesting that placental insufficiency caused by the loss of UTF1 expression in extra-embryonic ectodermal cells at least in part contributed to this phenotype. We also found that the effects of loss of UTF1 expression in embryonic stem cells on their pluripotency were very subtle. Genome structure and sequence comparisons revealed that the UTF1 gene exists only in placental mammals. Our analyses of a family of genes with homology to UTF1 revealed a possible mechanism by which placental mammals have evolved the UTF1 genes.

【 授权许可】

CC BY