Role of NOD2 and RIP2 in host–microbe interactions with Gram-negative bacteria: insights from the periodontal disease model:

Innate Immunity

Joao ACSouza¹ 

关键词: Bone resorption;    inflammation;    innate immunity;    macrophages;    NOD2 signaling adaptor protein;   

DOI  :  10.1177/1753425916666652

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

NOD2 is a member of the NLR family of proteins that participate in the activation of the innate immune response. RIP2 is a downstream kinase activated by both NOD1 and NOD2. There is scarcity of information regarding the relevance of NOD2 in periodontitis, a chronic inflammatory condition characterized by inflammatory bone resorption. We used NOD2-KO and RIP2-KO mice in a model of microbial-induced periodontitis. Heat-killed Aggregatibacter actinomycetemcomitans was injected in the gingival tissues three times/wk for 4 wk. Bone resorption was assessed by μCT analysis; osteoclasts were identified by immunohistochemical staining for TRAP and inflammation was assessed using a severity score system in H/E-stained sections. In vitro studies using primary macrophages assessed the response macrophages using qPCR-based array and multi-ligand ELISA. Bone resorption and osteoclastogenesis were significantly reduced in NOD2-KO mice. Severity of inflammation was not affected. qPCR-focused arrays and multi-ligand ELIS...

【 授权许可】

CC BY