Prolonged Graft Survival in Older Recipient Mice Is Determined by Impaired Effector T-Cell but Intact Regulatory T-Cell Responses

PLoS One

Christian Denecke¹  Xupeng Ge¹  Damanpreet Singh Bedi¹  Stefan G. Tullius¹  Irene Kyung-eun Kim¹  Anke Jurisch¹  Anne Weiland¹  Xian C. Li²  Antje Habicht³ 

[1] Division of Transplant Surgery and Transplant Surgery Research Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America;Transplant Research Center, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America;Transplantation Research Center, Brigham and Women's Hospital and Children's Hospital of Boston, Boston, Massachusetts, United States of America

关键词: T cells;    Skin grafting;    Transplant rejection;    Immune response;    Memory T cells;    Cytokines;    Spleen;    Enzyme-linked immunoassays;   

DOI  :  10.1371/journal.pone.0009232

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Elderly organ transplant recipients represent a fast growing segment of patients on the waiting list. We examined age-dependent CD4+ T-cell functions in a wild-type (WT) and a transgenic mouse transplant model and analyzed the suppressive function of old regulatory T-cells. We found that splenocytes of naïve old B6 mice contained significantly higher frequencies of T-cells with an effector/memory phenotype (CD4+CD44highCD62Llow). However, in-vitro proliferation (MLR) and IFNγ-production (ELISPOT) were markedly reduced with increasing age. Likewise, skin graft rejection was significantly delayed in older recipients and fewer graft infiltrating CD4+T-cells were observed. Old CD4+ T-cells demonstrated a significant impaired responsiveness as indicated by diminished proliferation and activation. In contrast, old alloantigen-specific CD4+CD25+FoxP3+ T-cells demonstrated a dose-dependent well-preserved suppressor function. Next, we examined characteristics of 18-month old alloreactive T-cells in a transgenic adoptive transfer model. Adoptively transferred old T-cells proliferated significantly less in response to antigen. Skin graft rejection was significantly delayed in older recipients, and graft infiltrating cells were reduced. In summary, advanced recipient age was associated with delayed acute rejection and impaired CD4+ T-cell function and proliferation while CD4+CD25+FoxP3+ T-cells (Tregs) showed a well-preserved function.

【 授权许可】

CC BY