Endotoxin recognition in fish results in inflammatory cytokine secretion not gene expression:

Innate Immunity

NereaRoher¹ 

关键词: macrophage;    cytokines;    lipopolysaccharide;    signal transduction;    comparative immunology/evolution;   

DOI  :  10.1177/1753425909348232

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Macrophages are phagocytes that have a central role in the organization of the immune system after an infection. These cells can recognize specific molecular components of micro-organisms (pathogen-associated molecular patterns, PAMPs) via specific receptors (PRRs) and elicit specific cellular responses. In the past, the expression of immune genes in response to different PAMPs has been characterized in different fish species. However, little is known about actual cytokine release. We characterized the secretion of tumour necrosis factor (TNF)-α in primary macrophage cultures of rainbow trout (Oncorhynchus mykiss) in response to several PAMPs by Western blot and compared this to the induction of TNF-α gene expression as well as other pro-inflammatory cytokines such as interleukin (IL)-1β and IL-6 and anti-viral molecules such as INF-α and Mx protein (Mx). We show that lipopolysaccharide (LPS) and zymosan are major inducers of TNF-α secretion, which is not initially linked to the induction of TNF-α mRNA ex...

【 授权许可】

CC BY