Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice:

Innate Immunity

JenniferNicholas¹ 

关键词: Inflammation;    macrophage;    microarray;    muscle recovery;    neutrophil;   

DOI  :  10.1177/1753425914527326

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

Innate pro-inflammatory processes, such as chemokine signaling and leukocyte infiltration, predominate during the first 48 h after an acute skeletal muscle injury. However, the time course of chemokine expression and its relationship to leukocyte infiltration after acute muscle injury within this early post-injury time period has not been investigated. In this study, 46 anesthetized female C57BL/6NHsd mice underwent a closed crush injury of the gastrocnemius muscle and were euthanized 4, 8, 24 and 48 h post-injury. Microarray analysis found 14 chemokine genes to be up-regulated during this period, 12 of which are involved in macrophage or neutrophil chemotaxis, with up-regulation peaking at either 8 or 48 h. RT-PCR analysis on select chemokines confirmed the microarray activation pattern. Neutrophil infiltration patterns mirrored the time course of neutrophil-related chemokines with Gr-1-, 1A8- and 7/4-positive neutrophils infiltrating the muscle 4 h after injury, decreasing at 48 h. Conversely, gene expr...

【 授权许可】

CC BY