The Glycosyltransferase Repertoire of the Spikemoss Selaginella moellendorffii and a Comparative Study of Its Cell Wall

PLoS One

William G. T. Willats¹  Alison Roberts²  Henrik Vibe Scheller²  Jesper Harholt²  Iben Sørensen²  Jonatan Fangel³  Peter Ulvskov⁴  Bent Larsen Petersen⁵  Jo Ann Banks⁶ 

[1] Department of Biological Sciences, University of Rhode Island, Kingston, Rhode Island, United States of America;Department of Plant Biology and Biotechnology, VKR Research Centre “Pro-Active Plants,” University of Copenhagen, Frederiksberg, Denmark;Department of Plant Biology, Cornell University, Ithaca, New York, United States of America;Department of Plant and Microbial Biology, University of California, Berkeley, California, United States of America;Feedstocks Division, Joint Bioenergy Institute, Emeryville, California, United States of America;Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America

关键词: Plant cell walls;    Arabidopsis thaliana;    Biosynthesis;    Phylogenetic analysis;    Rice;    Flowering plants;    Algae;    Polymers;   

DOI  :  10.1371/journal.pone.0035846

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Spike mosses are among the most basal vascular plants, and one species, Selaginella moellendorffii, was recently selected for full genome sequencing by the Joint Genome Institute (JGI). Glycosyltransferases (GTs) are involved in many aspects of a plant life, including cell wall biosynthesis, protein glycosylation, primary and secondary metabolism. Here, we present a comparative study of the S. moellendorffii genome across 92 GT families and an additional family (DUF266) likely to include GTs. The study encompasses the moss Physcomitrella patens, a non-vascular land plant, while rice and Arabidopsis represent commelinid and non-commelinid seed plants. Analysis of the subset of GT-families particularly relevant to cell wall polysaccharide biosynthesis was complemented by a detailed analysis of S. moellendorffii cell walls. The S. moellendorffii cell wall contains many of the same components as seed plant cell walls, but appears to differ somewhat in its detailed architecture. The S. moellendorffii genome encodes fewer GTs (287 GTs including DUF266s) than the reference genomes. In a few families, notably GT51 and GT78, S. moellendorffii GTs have no higher plant orthologs, but in most families S. moellendorffii GTs have clear orthologies with Arabidopsis and rice. A gene naming convention of GTs is proposed which takes orthologies and GT-family membership into account. The evolutionary significance of apparently modern and ancient traits in S. moellendorffii is discussed, as is its use as a reference organism for functional annotation of GTs.

【 授权许可】

CC BY