Different innate immunity and clearance of Salmonella Pullorum in macrophages from White Leghorn and Tibetan Chickens:

European Journal of Inflammation

ShaoWei¹ 

关键词: immune response;    macrophages;    Salmonella Pullorum;    Tibetan Chickens;    White Leghorn;   

DOI  :  10.1177/2058739218780039

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

Salmonella enterica serovar Gallinarum biovar Pullorum (S. Pullorum) is responsible for the systemic salmonellosis in different breeds of chickens. Macrophages, as host cells, play a key role in the innate immune response following infection with S. Pullorum. In this study, we first generated macrophages from two breeds of chicken (White Leghorn (WL) and Tibetan Chickens (TC)) peripheral blood monocytes in vitro. Then, we showed that the production of interleukin-1β (IL-1β), macrophage inflammatory protein-1β (MIP-1β) and interleukin-10 (IL-10) in lipopolysaccharide (LPS)-treated macrophages was significantly higher compared with the unstimulated cells in TC. LPS triggered only more expression of IL-10 in WL macrophages. Furthermore, macrophages from TC eliminated intracellular bacteria more efficiently than those from WL after S. Pullorum infection at a multiplicity of infection (MOI) 1. In addition, the variation between individuals and sex had the crucial effect on the immune response to LPS and S. Pullorum invasion.

【 授权许可】

CC BY-NC