Anti-proliferative and anti-inflammatory activities of the sea cucumber Holothuria polii aqueous extract:

SAGE Open Medicine

MikeKareh¹ 

关键词: Sea cucumber;    Holothuria polii;    anti-proliferative;    anti-inflammatory;    breast cancer;   

DOI  :  10.1177/2050312118809541

学科分类：医学（综合）

来源: Sage Journals

【 摘 要 】

Objective:Sea cucumbers are considered among the most important functional foods. Following bioassay guided fractionation, we assessed the anti-proliferative and anti-inflammatory activities of Holothuria polii (H. polii) extracts.Methods:Sea cucumber ethanolic extract and the partially purified aqueous fractions were assessed for their anti-proliferative activities. These latter bioactivities were evaluated in the highly invasive MDA-MB-231 human breast cancer cells in two-dimensional and three-dimensional cultures using trypan blue exclusion assay. The tumor-suppressive effects of sea cucumber ethanolic extract and aqueous fractions were assayed by measuring the trans-well invasion of MDA-MB-231 cells and the expression of some epithelial mesenchymal transition markers using quantitative reverse-transcription polymerase chain reaction and western blot analysis. The anti-inflammatory activity of the aqueous fraction was tested by measuring the secreted levels of interleukin-6, nitric oxide, and matrix me...

【 授权许可】

CC BY