Isolation, Sequence Identification and Tissue Expression of a Novel Tobacco (Nicotiana Tabacum) Gene-GME1

Biochemistry and Biophysics

liu yonggang¹ 

关键词: Tobacco;    GME1;    Tissue Expression;   

DOI  :  

学科分类：生物化学/生物物理

来源: Science and Engineering Publishing Company

【 摘 要 】

Abstract: The complete coding sequence of one tobacco (Nocotiana tabacum) gene-GDP-mannose 3',5'-epimerase (GME1), was amplified based on two tobacco ESTs. The complete coding sequence of tobacco GME1 gene was 1,131bp which encodes a protein of 376 amino acids. Sequence analysis revealed that the GME1 of tobacco shares high homology with the GME1 of lycopersicon esculentum (96%), peach (95%), castor bean (94%), malpighia glabra (94%), ribes nigrum (Accesession number: ACM90324, 93%), cucumber (92%), camellia sinensis (92%), soybean (92%). The expression profile was studied and the results indicated that tobacco GME1 gene was differentially expressed in detected tobacco tissues including leaf, stem, root and flower.

【 授权许可】

Unknown