Rapid identification of bioactive peptides with antioxidant activity from the enzymatic hydrolysate of Mactra veneriformis by UHPLC-Q-TOF mass spectrometry

卷:167

Liu, Rui ; Zheng, Wenwen ; Li, Jun ; Wang, Lingchong ; Wu, Hao ; Wang, Xinzhi ; Shi, Lei

Nanjing Univ Chinese Med

关键词: UHPLC-MS/MS;    Protein hydrolysates;    Peptides;    Antioxidant;    Mactra veneriformis;   

DOI  :  10.1016/j.foodchem.2014.06.113

学科分类：食品科学和技术

【 摘 要 】

Analysis of peptide components of protein hydrolysates is often difficult due to the lack of suitable analytical methods. In the present study, an UHPLC-Q-TOF MS/MS method was developed and used to identify peptides derived from the protein hydrolysate of Mactra venenformis. The peptide sequences were deduced by de novo sequencing based on MS/MS fragmentation data. A total of 21 peptides, four nucleobases, and one nucleoside were identified from the hydrolysate using this method. These peptides were chemically synthesised and showed antioxidant activity in radical scavenging assays. This method is suitable for quick, sensitive, and accurate analysis of complex protein hydrolysates. (C) 2014 Elsevier Ltd. All rights reserved.