A Method for Multiple Mycotoxin Analysis in Wines by Solid Phase Extraction and Multifunctional Cartridge Purification, and Ultra-High-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry

Toxins

Masayoshi Tamura¹  Ayumi Takahashi¹  Atsuo Uyama¹ 

[1] Research Laboratories for Food Safety Chemistry, Asahi Group Holdings, Ltd., 1-21, Midori 1, Moriya, Ibaraki 302-0106, Japan;

关键词: UHPLC-MS/MS;    mycotoxins;    wine;    solid phase extraction;    multiple analyses;   

DOI  :  10.3390/toxins4060476

来源: mdpi

【 摘 要 】

An analytical method using two solid phase extractions and ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was developed for the identification and quantification of 14 mycotoxins (patulin, deoxynivalenol, aflatoxins B₁, B₂, G₁, G₂, M₁, T-2 toxin, HT-2 toxin, zearalenone, fumonisins B₁, B₂, B₃, and ochratoxin A) in domestic and imported wines. Mycotoxins were purified with an Oasis HLB cartridge, followed by a MultiSep^TM #229 Ochra. As a result, sufficient removal of the pigments and highly polar matrices from the red wines was achieved. UHPLC conditions were optimized, and 14 mycotoxins were separated in a total of 13 min. Determinations performed using this method produced high correlation coefficients for the 14 mycotoxins (R > 0.990) and recovery rates ranging from 76 to 105% with good repeatability (relative standard deviation RSD < 12%). Twenty-seven samples of domestic and imported wines were analyzed using this method. Although ochratoxin A (OTA) and fumonisins (FMs) were detected in several samples, the FM levels were less than limits of quantification (LOQs) (1 μg/L), and even the largest of the OTA levels was below the EU regulatory level (2 μg/L). These results suggest that the health risk posed to consumers from the wines available in Japan is relatively low.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.