Molecular cloning and tissue mRNA levels of 15 genes involved in lipid metabolism in Synechogobius hasta

卷:117

Chen, Qi-Liang ; Luo, Zhi ; Huang, Chao ; Zheng, Jia-Lang ; Pan, Ya-Xiong ; Song, Yu-Feng ; Hu, Wei

关键词: Lipid metabolism;    Molecular cloning;    Synechogobius hasta;    Tissue mRNA levels;   

DOI  :  10.1002/ejlt.201400164

学科分类：食品科学和技术

【 摘 要 】

The regulation of lipid metabolism is complex, and is currently an extensive area of research. In this study, the partial sequences of 15 genes involved in lipid metabolism were cloned from the liver of Synechogobius hasta, including g6pd, 6pgd, me, icdh, fas, acc, acc, lpl, atgl, hsla, hslb, cpt 1a, srebp-1, ppar, and ppar. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of S. hasta. mRNA of all genes was detected in the liver, spleen, muscle, gill, brain, intestine, and heart, but at varying levels. Practical applications: Excessive fat accumulation and disordered lipid metabolism have become serious problems in the sustainable and healthy development of aquaculture. The present study facilitates studies on the regulation of lipid metabolism at the molecular level in fish. The tissue expression profiles of genes increase our understanding of their physiological roles.