期刊论文详细信息
卷:176
Highly sensitive electrochemical impedance spectroscopy immunosensor for the detection of AFB(1) in olive oil
Yu, Lili ; Zhang, Yang ; Hu, Chenyi ; Wu, Hui ; Yang, Yayun ; Huang, Chusen ; Jia, Nengqin
关键词: Immunosensor;    Carbon nanotube;    Room temperature ionic liquids (RTILs);    Aflatoxin B-1 (AFB(1));    Olive oil;    Electrochemical impedance spectroscopy (EIS);   
DOI  :  10.1016/j.foodchem.2014.12.030
学科分类:食品科学和技术
PDF
【 摘 要 】

Aflatoxin produced by Aspergillus flavus and Aspergillus parasiticus are commonly found in olive and its derivatives. Aflatoxin B-1 (AFB(1)) is a predominant toxin detected abundantly and has been implicated in the etiology of human hepatocellular carcinoma. This study proposes a sensitive and convenient electrochemical impedance spectroscopy (EIS) method for determining AFB(1) by MWCNTs/RTIL composite films-based immunosensor. The calibration curve for AFB(1) was linear in the range of 0.1-10 ng mL(-1) with the limit of detection (LOD) 0.03 ng mL(-1). The presence of MWCNTs warrant fast electron transfer, and the ionic liquid provides a benign microenvironment for antibody. The experimental parameters, such as pH and incubating time, have been investigated and optimized. Furthermore, the detection of AFB(1) is presented to test this method after extracted from olive oils. It can be anticipated that this method would be used for the detection of AFB1 in various agriculture products and vegetable oils. (C) 2014 Elsevier Ltd. All rights reserved.

【 授权许可】

   

【 预 览 】
附件列表
Files Size Format View
JA201706070005292SK.pdf KB PDF download
  文献评价指标  
  下载次数:17次 浏览次数:35次