A-type ECG and EGCG dimers disturb the structure of 3T3-L1 cell membrane and strongly inhibit its differentiation by targeting peroxisome proliferator-activated receptor gamma with miR-27 involved mechanism

卷:26

Zhu, Wei ; Zou, Bo ; Nie, Rongzu ; Zhang, Ying ; Li, Chun-mei

关键词: Adipogenesis;    PPAR gamma;    MicroRNAs;    Structural-dependent;    Membrane disturbing effects;   

DOI  :  10.1016/j.jnutbio.2015.05.006

学科分类：食品科学和技术

【 摘 要 】

The effects of four proanthocyanidin dimers including epicatechin-(4 beta -> 8, 2 beta -> O -> 7)-epicatechin (A-type EC dimer), epicatechin-(4 beta -> 8)-epicatechin (B-type EC dimer), epicatechin-3-gallate-(4 beta -> 8, 2 beta -> O -> 7)-epicatechin-3-gallate (A-type ECG dimer) and epigallocatechin-3-gallate-(4 beta -> 8, 2 beta -> O -> 7)-epigallocatechin-3-gallate (A-type EGCG dimer) on 3T3-L1 preadipocyte cell differentiation and the underlying mechanisms were explored and compared. The results showed that A-type ECG dimer and A-type EGCG dimer significantly reduced the intracellular lipid accumulation in 3T3-L1 preadipocyte cells by targeting miR-27a and miR-27b as well as peroxisome proliferator-activated receptor la (PPAR gamma) in the early stage of differentiation, while A-type EC dimer and B-type EC dimer showed little effect. In addition, our results revealed that the inhibitory effects of proanthocyanidin dimers on 3T3-L1 preadipocyte differentiation were highly structure-dependent and the effects were associated with the dimer-membrane interactions. The presence of galloyl moieties and A-type linkage within the structure of proanthocyanidins might be crucial for their inhibitory effect on adipogenesis. The strong disturbing effects of A-type ECG and A type EGCG dimers on the fluidity, hydrophobicity and permeability of membrane of 3T3-L1 preadipocyte cell were at least, in part, responsible for their distinct inhibitory effects on adipocyte hyperplasia. (C) 2015 Elsevier Inc. All rights reserved.