Gene expression and protein secretion during human mesenchymal cell differentiation into adipogenic cells

BMC Cell Biology

Radovan Borojevic¹  José Mauro Granjeiro³  Rosana Bizon Vieira Carias³  Marcus Vinicius Telles Teixeira³  Paola Romina Amable² 

[1] Faculdade de Medicina de Petrópolis, Faculdades Arthur Sá Earp Neto, Petrópolis, Rio de Janeiro, Brazil;Excellion Biomedical Services S.A., Rua Afrânio de Mello Franco 333, Quitandinha, Petrópolis, Rio de Janeiro, Brazil;National Institute of Metrology, Quality and Technology (Inmetro), Rio de Janeiro, Xerém, Brazil

关键词: Protein quantification;    qPCR;    Adipogenesis;    Human mesenchymal stromal cells;   

Others  :  1088694
DOI  :  10.1186/s12860-014-0046-0

 received in 2014-07-01, accepted in 2014-12-11,  发布年份 2014

【 摘 要 】

Background

Mesenchymal stromal cells (MSC) can be obtained from potentially any tissue from the human body, but cells purified from different sources are undoubtedly different, and for each medical application, the MSC with the best regenerative potential should be chosen.

Results

Bone marrow-derived mesenchymal stromal cells (BM-MSC), adipose tissue-derived mesenchymal stromal cells (AT-MSC) and Wharton’s Jelly-derived mesenchymal stromal cells (WJ-MSC) were isolated from human tissues and were cultured under differentiation media supplemented with fetal bovine serum. We quantified the expression of stem cell and adipocyte genetic markers using quantitative real time PCR, as well as the secretion of cytokines, extracellular matrix components and growth factors using Luminex and ELISA. All three MSC differentiated into adipogenic cells. AT-MSC showed the highest shift in ADIPOQ, CEBPA and PPARG mRNA expression. BM-MSC kept high expression levels of stem-cell markers SOX2 and POU5F1. WJ-MSC showed the lowest increase in mRNA expression when cells were induced to differentiate into adipocytes. Regarding protein secretion, adipocyte-like cells generated from WJ-MSC secreted the highest chemokine levels. AT-MSC-derived adipocyte-like cells secreted the lowest cytokine amounts and the highest quantity of collagen types I and III. Adipocyte-like cells obtained from BM-MSC secreted high amounts of most angiogenic factors, growth factors TGF-β1 and TGF-β2, collagens type II and IV, heparan sulfate, laminin and aggrecan.

Conclusion

Mesenchymal stromal cells purified from different tissues have a different behavior when induced to differentiate into adipocyte-like cells.

【 授权许可】

   
2014 Amable et al.; licensee BioMed Central.

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