Application of HPLC-DAD after SPE/QuEChERS with ZrO2-based sorbent in d-SPE clean-up step for pesticide analysis in edible oils

卷:190

Tuzimski, Tomasz ; Rejczak, Tomasz

Med Univ Lublin

关键词: QuEChERS;    Solid phase extraction (SPE);    Dispersive-SPE;    Zirconium dioxide-based sorbent;    Pesticides;    HPLC-DAD;    Edible oils;    Matrix effect;   

DOI  :  10.1016/j.foodchem.2015.05.072

学科分类：食品科学和技术

【 摘 要 】

In this study, the solid-phase extraction/quick, easy, cheap, effective, rugged and safe (SPE/QuEChERS) technique was adapted to develop a simple sample treatment for multi-residue pesticide analysis of edible oils. The proposed method is based on liquid liquid partitioning with acetonitrile followed by dispersive solid phase extraction using zirconia-coated silica particles for extract purification. To evaluate the described method, 21 pesticides belonging to different chemical classes were analysed using high performance liquid chromatography with diode-array detection (HPLC DAD). For validation purposes, recovery studies were performed at 75 ng g(-1), 125 ng g(-1), 250 ng g(-1), 500 ng g(-1) and 1000 ng g(-1) levels. Recoveries were over the range of 50-130% for most of the analytes, with relative standard deviations less than 15% being observed. HPLC DAD provided suitable linearity, precision and accuracy. The validated method was successfully applied to the analysis of edible oil samples selected from the market. (C) 2015 Elsevier Ltd. All rights reserved.