Yarrowia lipolytica as a biotechnological chassis to produce usual and unusual fatty acids

卷:61

Ledesma-Amaro, Rodrigo ; Nicaud, Jean-Marc

INRA AgroParisTech

关键词: Yarrowia lipolytica;    Lipid metabolism;    Oleaginous yeasts;    Unusual fatty acids;    Systems metabolic engineering;   

DOI  :  10.1016/j.plipres.2015.12.001

学科分类：食品科学和技术

【 摘 要 】

One of the most promising alternatives to petroleum for the production of fuels and chemicals is bio-oil based chemistry. Microbial oils are gaining importance because they can be engineered to accumulate lipids enriched in desired fatty acids. These specific lipids are closer to the commercialized product, therefore reducing pollutants and costly chemical steps. Yarrowia lipolytica is the most widely studied and engineered oleaginous yeast. Different molecular and bioinformatics tools permit systems metabolic engineering strategies in this yeast, which can produce usual and unusual fatty acids. Usual fatty acids, those usually found in triacylglycerol, accumulate through the action of several pathways, such as fatty acid/triacylglycerol synthesis, transport and degradation. Unusual fatty acids are enzymatic modifications of usual fatty acids to produce compounds that are not naturally synthetized in the host. Recently, the metabolic engineering of microorganisms has produced different unusual fatty acids, such as building block ricinoleic acid and nutraceuticals such as conjugated linoleic acid or polyunsaturated fatty acids. Additionally, microbial sources are preferred hosts,for the production of fatty acid-derived compounds such as gamma-decalactone, hexanal and dicarboxylic acids. The variety of lipids produced by oleaginous microorganisms is expected to rise in the coming years to cope with the increasing demand. (C) 2015 The Authors. Published by Elsevier Ltd.