Use of TD-GC-TOF-MS to assess volatile composition during post-harvest storage in seven accessions of rocket salad (Eruca sativa)

卷:194

Bell, Luke ; Spadafora, Natasha D. ; Mueller, Carsten T. ; Wagstaff, Carol ; Rogers, Hilary J.

Univ Reading

关键词: Eruca sativa;    Diplotaxis tenuifolia;    Brassicaceae;    Rocket salad;    Rucola;    Gas chromatography mass spectrometry;    Volatile organic chemicals;    Isothiocyanates;   

DOI  :  10.1016/j.foodchem.2015.08.043

学科分类：食品科学和技术

【 摘 要 】

An important step in breeding for nutritionally enhanced varieties is determining the effects of the post-harvest supply chain on phytochemicals and the changes in VOCs produced over time. TD-GC-TOF-MS was used and a technique for the extraction of VOCs from the headspace using portable tubes is described. Forty-two compounds were detected; 39 were identified by comparison to NIST libraries. Thirty-five compounds had not been previously reported in Eruca sativa. Seven accessions were assessed for changes in headspace VOCs over 7 days. Relative amounts of VOCs across 3 time points were significantly different - isothiocyanate-containing molecules being abundant on 'Day 0'. Each accession showed differences in proportions/types of volatiles produced on each day. PCA revealed a separation of VOC profiles according to the day of sampling. Changes in VOC profiles over time could provide a tool for assessment of shelf life. (C) 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).