Selective separation and concentration of antihypertensive peptides from rapeseed protein hydrolysate by electrodialysis with ultrafiltration membranes

卷:197

He, Rong ; Girgih, Abraham T. ; Rozoy, Elodie ; Bazinet, Laurent ; Ju, Xing-Rong ; Aluko, Rotimi E.

Univ Manitoba

关键词: Rapeseed;    Protein hydrolysate;    Electrodialysis with ultrafiltration membrane;    ACE;    Renin;    Spontaneously hypertensive rats;    Systolic blood pressure;   

DOI  :  10.1016/j.foodchem.2015.11.081

学科分类：食品科学和技术

【 摘 要 】

Rapeseed protein isolate was subjected to alcalase digestion to obtain a protein hydrolysate that was separated into peptide fractions using electrodialysis with ultrafiltration membrane (EDUF) technology. The EDUF process (6 h duration) led to isolation of three peptide fractions: anionic (recovered in KCl-1 compartment), cationic (recovered in KCl-2 compartment), and those that remained in the feed compartment, which was labeled final rapeseed protein hydrolysate (FRPH). As expected the KCl-1 peptides were enriched in negatively-charged (43.57%) while KCl-2 contained high contents of positively-charged (28.35%) amino acids. All the samples inhibited angiotensin converting enzyme (ACE) and renin activities in dose-dependent manner with original rapeseed protein hydrolysate having the least ACE-inhibitory IC50 value of 0.0932 +/- 0.0037 mg/mL while FRPH and KCl-2 had least renin-inhibitory IC50 values of 0.47 +/- 0.05 and 0.55 +/- 0.06 mg/mL, respectively. Six hours after oral administration (100 mg/kg body weight) to spontaneously hypertensive rats, the FRPH produced the maximum systolic blood pressure reduction of -51 mmHg. (C) 2015 Elsevier Ltd. All rights reserved.