Moderately increased maternal dietary energy intake delays foetal skeletal muscle differentiation and maturity in pigs

卷:55

Zou, Tiande ; He, Dongting ; Yu, Bing ; Yu, Jie ; Mao, Xiangbing ; Zheng, Ping ; He, Jun ; Huang, Zhiqing ; Shu, Yan ; Liu, Yue ; Chen, Daiwen

Sichuan Agr Univ

关键词: Energy intake;    Gestation;    Longissimus;    Foetus;    Myogenesis;    Pig;   

DOI  :  10.1007/s00394-015-0996-9

学科分类：食品科学和技术

【 摘 要 】

Objectives This study aimed to evaluate the effects of moderately increased maternal dietary energy intake during gestation on foetal skeletal muscle development and metabolism with pig as a model. Methods Twelve primiparous purebred Large White sows (initial body weight 135.5 +/- 1.6 kg) were allocated to one of two energy intake treatments: normal-energy-intake group (Con, 30.96 MJ DE/day) as recommended by the National Research Council (NRC; 2012) and high-energy-intake group (HE, 34.15 MJ DE/day). The nutritional treatments were introduced from mating to day 90 of gestation. On day 90 of gestation, foetuses were examined by morphological, biochemical and molecular analysis of the longissimus muscle. Umbilical vein serum hormones were measured. Results Sow body weight was increased in HE group compared with Con group (P < 0.05), whereas foetal myofibre density was decreased (P < 0.05). Meanwhile, protein concentration, creatine kinase and lactate dehydrogenase activities and umbilical vein serum triiodothyronine (T3) concentration were decreased in HE foetuses (P < 0.05). Maternal HE diets decreased the mRNA abundance of muscle growth-related genes, myosin heavy-chain (MYH/MyHC) genes (MYH2 and MYH1) and insulin-like growth factor 1 and insulin growth factor-binding protein 5 (P < 0.05). Furthermore, the protein expressions of myogenic differentiation factor 1, myogenin and fast-MyHC isoforms were reduced in HE foetuses (P < 0.05). Conclusion Our results suggest that moderately increased maternal dietary energy intake delays the differentiation and maturation in skeletal muscle of the foetus on day 90 of gestation.