Ultra-sensitive biosensor based on genetically engineered acetylcholinesterase immobilized in poly (vinyl alcohol)/Fe-Ni alloy nanocomposite for phosmet detection in olive oil

卷:203

El-Moghazy, A. Y. ; Soliman, E. A. ; Ibrahim, H. Z. ; Noguer, T. ; Marty, J. -L. ; Istamboulie, G.

Univ Perpignan

关键词: Biosensor;    Acetylcholinesterase;    Nanocomposite;    Organophosphorus pesticides;    Screen-printed electrodes;    Olive oil;   

DOI  :  10.1016/j.foodchem.2016.02.014

学科分类：食品科学和技术

【 摘 要 】

An ultra-sensitive screen-printed biosensor was successfully developed for phosmet detection in olive oil, based on a genetically-engineered acetylcholinesterase (AChE) immobilized in a azide-unit waterpendant polyvinyl alcohol (PVA-AWP)/Fe-Ni alloy nanocomposite. Fe-Ni not only allowed amplifying the response current but also lowering the applied potential from 80 mV to 30 mV vs Ag/AgCl. The biosensor showed a very good analytical performance for phosmet detection, with a detection limit of 0.1 nM. This detection limit is lower than the allowable concentrations set by international regulations. In addition to the good reproducibility, operational and storage stability, the developed biosensor was successfully used for the determination of phosmet in olive oil samples without any laborious pretreatment. The phosmet recovery rate was about 96% after a simple liquid-liquid extraction. (c) 2016 Elsevier Ltd. All rights reserved.