Environmental Enteric Dysfunction Is Associated With Poor Linear Growth and Can Be Identified by Host Fecal mRNAs

卷:63

Ordiz, Maria Isabel ; Shaikh, Nurmohammad ; Trehan, Indi ; Maleta, Ken ; Stauber, Jennifer ; Shulman, Robert ; Devaraj, Sridevi ; Tarr, Phillip I. ; Manary, Mark J.

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关键词: droplet digital PCR;    dual sugar absorption test;    environmental enteric dysfunction;    host fecal transcripts;    random forest modeling;    stunting;   

DOI  :  10.1097/MPG.0000000000001315

学科分类：食品科学和技术

【 摘 要 】

Objective:Environmental enteric dysfunction (EED) can be assessed by the lactulose:mannitol (L:M) test. Our objective was to determine if selected host fecal transcripts were correlated with EED, and whether transcripts and clinical characteristics could be used to predict EED in rural African children.Methods:Demographic and sanitation characteristics, along with L:M testing and host fecal transcript analyses from 798 asymptomatic Malawian children aged 12 to 61 months were compared with linear growth over the subsequent 3 months. Fecal host mRNA analysis included quantification of expression of 18 transcripts associated with L:M. Permeability was categorized as normal (L:M 0.15), moderate (0.15