Simple and rapid method for routine screening of bovine leukemia virus by loop-mediated isothermal amplification assay

The Journal of Veterinary Medical Science

Michiko MIYAMATO-HAYASHI¹  Yasuo INOSHIMA²  Yuka OKUWA³  Takaichi TANAKA³  Yuji HAYAKAWA³ 

[1] Ishikawa Agriculture and Forestry Research Center, Livestock Experiment Station, 93-2 Na, Tsuboyama, Houdatsushimizu, Hakui, Ishikawa 929-1325, Japan;Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan;Nanbu Livestock Hygiene Service Center, 324-2 Bo, Saida-machi, Kanazawa, Ishikawa 920-3101, Japan

关键词: blood DNA;    bovine leukemia virus;    cattle;    loop-mediated isothermal amplification;    screening;   

DOI  :  10.1292/jvms.16-0328

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Various techniques for screening and detection of bovine leukemia virus (BLV) were compared to ascertain a rapid and simple technique for routine examination. The performance of real-time PCR, nested PCR and loop-mediated isothermal amplification (LAMP) assays was compared using DNA extracted from whole blood instead of white blood cells (WBCs) of 23 cattle. Real-time PCR, LAMP and nested PCR detected 18, 16 and 11 BLV-positive cattle, respectively. These results suggest that LAMP using DNA from whole blood could enable rapid examination, as isolation of WBCs and electrophoresis is time-consuming and could be useful as a simple and rapid method for routine screening of BLV.

【 授权许可】

Unknown