Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines - ScienceDirect

Stem Cell Research

Yixiang Zhong¹ 

关键词: Stem cells;    Blastocyst;    Mice;    Rats;    Extraembryonic endoderm;    ExEn;    extraembryonic endoderm;    LIF;    leukemia inhibitory factor;    MEF;    mouse embryo fibroblasts;    PDGF;    platelet-derived growth factor;    PrE;    primitive endoderm;    pXEN cells;    primitive (mouse) ExEn stem cells;    XEN cells;    (mouse) ExEn stem cells;    XENP cells;    (rat) ExEn stem cells;   

DOI  :  10.1016/j.scr.2018.05.008

学科分类：生物科学（综合）

来源: Elsevier

【 摘 要 】

Mouse blastocysts contain the committed precursors of the extraembryonic endoderm (ExEn), which express the key transcription factor Oct4, depend on LIF/LIF-like factor-driven Jak/Stat signaling, and initially exhibit lineage plasticity. Previously described rat blastocyst-derived ExEn precursor-like cell lines (XENP cells/HypoSCs) also show these features, but equivalent mouse blastocyst-derived cell lines are lacking. We now present mouse blastocyst-derived cell lines, named primitive XEN (pXEN) cells, which share these and additional characteristics with the XENP cells/HypoSCs, but not with previously known mouse blastocyst-derived XEN cell lines. Otherwise, pXEN cells are highly similar to XEN cells by morphology, lineage-intrinsic differentiation potential, and multi-gene expression profile, although the pXEN cell profile correlates better with the blastocyst stage. Finally, we show that pXEN cells easily convert into XEN-like cells but not vice versa. The findings indicate that (i) pXEN cells are more representative than XEN cells of the blastocyst stage; (ii) mouse pXEN, rather than XEN, cells are homologs of rat XENP cells/HypoSCs, which we propose to call rat pXEN cells.

【 授权许可】

Unknown