Evaluation of the addition of glycerol to Cupriavidus necator culture medium over Poly(3-hydroxybutyrate) production

Materia

Furigo Junior, Agenor¹  Apati, Giannini Paszinick²  Schneider, Andrea Lima dos Santos²  Pezzin, Ana Paula Testa²  Kelbert, Maikon²  Garcia, Michele Cristina Formolo²  Sombrio, Bruna Regina² 

[1] Federal University of Santa Catarina, Florianópolis, Brazil;University of the Joinville Region, Joinville, Brazil

关键词: Poly(3-hydroxybutyrate);    Cupriavidus necator;    glycerol.;   

DOI  :  10.1590/s1517-707620170005.0243

学科分类：工程和技术（综合）

来源: Universidade Federal do Rio de Janeiro * Coordenacao dos Programas de Pos-Graduacao de Engenharia

【 摘 要 】

Glycerol was used as a source of additional carbon in the production of Poly(3-hydroxybutyrate) (P(3HB)). The inverted sugar and glycerol concentrations and the temperature of the Cupriavidus necator culture medium were evaluated using a Central Composite Rotational Design (CCRD). The results showed that the increase in temperature and sugar concentration led to an increase in production and P(3HB) accumulation and when 15 g L-1 of glycerol was added better results were obtained, however these were not considered statistically significant. The best results were obtained at 38 °C and with 30 g L-1 of inverted sugar. Although not considered statistically significant, the addition of 15 g L-1 of glycerol increased the P(3HB) accumulation percentage by 15 %, thus in kinetic terms, greater productivity was obtained in 0.32 g L-1 h-1 polymer. Larger scale assays are being conducted to verify if the addition of glycerol improves the thermal and mechanical properties of the synthesized polymer.

【 授权许可】

CC BY