The Journal of Veterinary Medical Science | |
Aldehyde dehydrogenase activity identifies a subpopulation of canine adipose-derived stem cells with higher differentiation potential | |
Tomoya HARAGUCHI1  Harumichi ITOH1  Shimpei NISHIKAWA1  Yu ARIKAWA1  Kazuhito ITAMOTO1  Yoshiki ITOH2  Toshie ISERI2  Munekazu NAKAICHI2  Masato HIYAMA3  Shotaro ETO3  Kenji TANI3  Yasuho TAURA3  | |
[1] Department of Small Animal Clinical Science, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8515, Japan;Department of Veterinary Radiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8515, Japan;Department of Veterinary Surgery, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8515, Japan | |
关键词: adipose-derived stem cells; aldehyde dehydrogenase activity; flow cytometry; osteogenic differentiation; adipogenic differentiation; | |
DOI : 10.1292/jvms.16-0503 | |
学科分类:兽医学 | |
来源: Japanese Society of Veterinary Science | |
【 摘 要 】
Adipose-derived stem cells (ADSCs) are abundant and readily obtained, and have been studied for their clinical applicability in regenerative medicine. Some surface antigens have been identified as markers of different ADSC subpopulations in mice and humans. However, it is unclear whether functionally distinct subpopulations exist in dogs. To address this issue, we evaluated aldehyde dehydrogenase (ALDH) activityâa widely used stem cell marker in mice and humansâby flow cytometry. Approximately 20% of bulk ADSCs showed high ALDH activity. Compared to cells with low activity (ALDHLo), the high-activity (ALDHHi) subpopulation exhibited a higher capacity for adipogenic and osteogenic differentiation. This is the first report of distinct ADSC subpopulations in dogs that differ in terms of adipogenic and osteogenic differentiation potential.
【 授权许可】
Unknown
【 预 览 】
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