期刊论文详细信息
The Journal of Veterinary Medical Science
Aldehyde dehydrogenase activity identifies a subpopulation of canine adipose-derived stem cells with higher differentiation potential
Tomoya HARAGUCHI1  Harumichi ITOH1  Shimpei NISHIKAWA1  Yu ARIKAWA1  Kazuhito ITAMOTO1  Yoshiki ITOH2  Toshie ISERI2  Munekazu NAKAICHI2  Masato HIYAMA3  Shotaro ETO3  Kenji TANI3  Yasuho TAURA3 
[1] Department of Small Animal Clinical Science, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8515, Japan;Department of Veterinary Radiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8515, Japan;Department of Veterinary Surgery, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8515, Japan
关键词: adipose-derived stem cells;    aldehyde dehydrogenase activity;    flow cytometry;    osteogenic differentiation;    adipogenic differentiation;   
DOI  :  10.1292/jvms.16-0503
学科分类:兽医学
来源: Japanese Society of Veterinary Science
PDF
【 摘 要 】

Adipose-derived stem cells (ADSCs) are abundant and readily obtained, and have been studied for their clinical applicability in regenerative medicine. Some surface antigens have been identified as markers of different ADSC subpopulations in mice and humans. However, it is unclear whether functionally distinct subpopulations exist in dogs. To address this issue, we evaluated aldehyde dehydrogenase (ALDH) activity—a widely used stem cell marker in mice and humans—by flow cytometry. Approximately 20% of bulk ADSCs showed high ALDH activity. Compared to cells with low activity (ALDHLo), the high-activity (ALDHHi) subpopulation exhibited a higher capacity for adipogenic and osteogenic differentiation. This is the first report of distinct ADSC subpopulations in dogs that differ in terms of adipogenic and osteogenic differentiation potential.

【 授权许可】

Unknown   

【 预 览 】
附件列表
Files Size Format View
RO201902197415335ZK.pdf 1563KB PDF download
  文献评价指标  
  下载次数:7次 浏览次数:7次