Bone regeneration using injectable poly (γ-benzyl-L-glutamate) microspheres loaded with adipose-derived stem cells in a mouse femoral non-union model

American Journal of Translational Research

Zhongyue Huang¹  Long Gao²  Xiaofan Yin³  Huijie Gu⁴ 

[1] Department of Orthopedics, Minhang Hospital, Fudan University, Shanghai 201199, China;Department of Orthopedics, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China;Department of Plastic Surgery, Beijing Shijitan Hospital Affiliated to Capital Medical University, Beijing 100038, China;Department of Polymer Materials, Shanghai University, Shanghai 200444, China

关键词: Microspheres;    poly (γ;    -benzyl-L-glutamate);    adipose-derived stem cells;    osteogenic differentiation;    bone regeneration;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Microspheres have gained immense popularity in bone tissue engineering because of their unique properties as injectable scaffolds for bone tissue regeneration. Herein, we evaluated the feasibility of using poly (γ-benzyl-L-glutamate) (PBLG) microspheres for bone engineering by examining the attachment, proliferation, and osteogenic differentiation of adipose-derived stem cells (ASCs) in vitro and the use of PBLG microspheres in healing non-union in vivo. Scanning electron microscopy and fluorescent 3, 30-dioctadecyloxacarbocyanine perchlorate-labeling were performed to study the attachment and growth of ASCs to the PBLG microspheres, and a DNA assay was performed to quantify cell proliferation with time. Osteogenic differentiation of ASCs cultured on the PBLG microspheres was assessed by determining alkaline phosphatase expression and extracellular calcium deposition, which was further confirmed by real-time polymerase chain reaction for osteogenesis-related genes. Femoral non-union were created in mouse models and filled with ASCs/PBLG microspheres in vivo. The results showed that ASCs attached, spread, and showed good osteogenic differentiation on the PBLG microspheres in vitro. Moreover, the ASCs/PBLG microspheres could repair the mouse femoral non-union in vivo. Thus, PBLG microspheres have good biocompatibility and cytocompatibility and are potentially useful as an injectable scaffolds for bone tissue engineering.

【 授权许可】

CC BY-NC