Blood ionized calcium levels and acute-phase blood glucose kinetics in goatsafter intramammary infusion of lipopolysaccharide

The Journal of Veterinary Medical Science

Naoki ISOBE¹  Miki OKITA¹  Kazuhiro KAWAI²  Yoshiki HAMAMOTO²  Reiichiro SATO²  Yasunori SHINOZUKA²  Akito HIGASHITANI² 

[1] Graduate School of Biosphere Science, HiroshimaUniversity, Higashi-Hiroshima, Hiroshima 739-8528, Japan;School of Veterinary Medicine, Azabu University,Sagamihara, Kanagawa 252-5201, Japan

关键词: glucose;    goat;    ionized calcium;    lipopolysaccharide;    mastitis;   

DOI  :  10.1292/jvms.17-0615

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

The aim of this study was to determine the blood ionized calcium (Ca) levels andacute-phase blood glucose kinetics in goats with mastitis induced by an intramammarychallenge of lipopolysaccharide (LPS). Five goats were subjected to intramammary challengeof either LPS (10 µg) or saline (control). Some clinical manifestations(rectal temperature, pulse rate, respiration rate, ruminal motility, physical activity,and dehydration) were observed, and blood was collected for the measurement of severalparameters [ionized and total Ca levels, blood glucose level, pH, and white blood count(WBC)] at 0 (just before challenge), 1–4, 6, 8, 12 and 24 hr post-challenge in both theLPS and control phases. Milk was collected at 0 (just before challenge), 4, 8, 12 and 24hr post-challenge to measure the somatic cell count (SCC) andN-acetyl-beta-D-glucosaminidase (NAGase) activity. In the LPS phase, increased rectaltemperature, significantly decreased ionized Ca and total Ca levels and WBCs were observedcompared with those at 0 hr, although there were no differences in all parameters betweenphases. LPS infusion significantly increased SCCs in milk and NAGase activity. The presentresults demonstrated that, during the acute phase of mastitis induced by intramammarychallenge by LPS at a concentration sufficient to cause general symptoms in goats, adecreased blood ionized Ca level occurs, but not hypoglycemia.

【 授权许可】

Unknown