Differential Effects of Resveratrol on the Expression of Brain-Derived Neurotrophic Factor Transcripts and Protein in the Hippocampus of Rat Brain

Iranian Journal of Medical Sciences

Mohammad Borji¹  Ali Akbar Owji¹  Sayed Mohammad Shafiee¹  Zahra Khoshdel²  Ghasem Ghasempour²  Shahla Shojaei³  Mohammad Reza Panjehshahin⁴ 

[1] Department of Biochemistry andRecombinant Protein Laboratory, Schoolof Medicine, Shiraz University of MedicalSciences, Shiraz, Iran;Department of Clinical Biochemistry,School of Pharmacy and PharmaceuticalSciences, Isfahan University of MedicalSciences, Isfahan, Iran;Department of Pharmacology, School ofMedicine, Shiraz University of MedicalSciences, Shiraz, Iran;Research Center for Psychiatry andBehavioral Sciences, Shiraz Universityof Medical Sciences, Shiraz, Iran

关键词: Resveratrol;    Brain-derived neurotrophic factor;    Hippocampus;    Rat;   

DOI  :  

学科分类：基础医学

来源: Shiraz University of Medical Sciences

【 摘 要 】

Background: The induction of brain-derived neurotrophic factor (BDNF) expression in the hippocampus has shown to play a role in the beneficial effects of resveratrol (RSV) on the learning and memory. The BDNF gene has a complicated structure with eight 5’ noncoding exons (I-IXa), each of which can splice to a common coding exon (IX) to form a functional transcript. Estrogens increase levels of BDNF transcripts in the hippocampus of rats. The aim of this study was to evaluate the effects of the phytoestrogen, RSV, on the splicing pattern of BDNF transcripts and on the pro-BDNF protein in the hippocampi of mother rats and their embryos. Methods: RSV (60 or 120 mg/kg BW/day) was administered orally to pregnant rats from days 1 to 20 of gestation. Hippocampi of adults and embryos were dissected 24 h after the last administration of RSV. Extracts from hippocampi were subject to quantitative (q) RT-PCR and Western blotting to assess splicing pattern of the BDNF transcripts and levels of pro-BDNF protein, respectively. Results: RSV (120 mg/kg BW/day) caused a statistically significant increase in the expression levels of BDNF exons III, IV and IX, but not the exon I in the hippocampi of adult rats (P≤0.05). Levels of pro-BDNF protein remained unchanged in the hippocampal tissues from both adult and embryonic rats treated by RSV (60 or 120 mg/kg BW/day). Conclusion: Our results showed that RSV differentially activates promoters of the BDNF gene in the hippocampus of pregnant rats, but fails to affect the pro-BDNF level neither in adult nor in the embryonic hippocampal tissues. 

【 授权许可】

CC BY