Stem Cell Research | |
Enhancing proliferation and optimizing the culture condition for human bone marrow stromal cells using hypoxia and fibroblast growth factor-2 - ScienceDirect | |
Jung-Seok Lee1  | |
关键词: Bone marrow stromal cells; Hypoxia; Fibroblast growth factor; Tissue regeneration; Microenvironment interactions; | |
DOI : 10.1016/j.scr.2018.01.010 | |
学科分类:生物科学(综合) | |
来源: Elsevier | |
【 摘 要 】
This study aimed to determine the cellular characteristics and behaviors of human bone marrow stromal cells (hBMSCs) expanded in media in a hypoxic or normoxic condition and with or without fibroblast growth factor-2 (FGF-2) treatment. hBMSCs isolated from the vertebral body and expanded in these four groups were evaluated for cellular proliferation/migration, colony-forming units, cell-surface characterization, in vitro differentiation, in vivo transplantation, and gene expression. Culturing hBMSCs using a particular environmental factor (hypoxia) and with the addition of FGF-2 increased the cellular proliferation rate while enhancing the regenerative potential, modulated the multipotency-related processes (enhanced chondrogenesis-related processes/osteogenesis, but reduced adipogenesis), and increased cellular migration and collagen formation. The gene expression levels in the experimental samples showed activation of the hypoxia-inducible factor-1 pathway and glycolysis in the hypoxic condition, with this not being affected by the addition of FGF-2. The concurrent application of hypoxia and FGF-2 could provide a favorable condition for culturing hBMSCs to be used in clinical applications associated with bone tissue engineering, due to the enhancement of cellular proliferation and regenerative potential.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201902194841861ZK.pdf | 4073KB | download |