Changes in skin structure of the Zip13-KO mouse by Makomo (Zizania latifolia) feeding

The Journal of Veterinary Medical Science

Kazushige TAKEHANA¹  Naoki TAKAHASHI²  Prasarn TANGKAWATTANA³  Jun MINAGUCHI⁴  Kaho SATO⁴  Takeshi YAMAUCHI⁴  Takeshi UENO⁴  Kenji IWAI⁴  Takuya HIROSE⁵ 

[1] Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand;Japan Institute of Leather Research, Toride, Ibaraki 302-0017, Japan;Laboratory of Mathematics, School of Food Science and Human Wellness, Rakuno Gakuen University Ebetsu, Hokkaido 069-8501, Japan;Laboratory of Microanatomy, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan;The Maple Foundation, Chuou-ku, Tokyo 103-0014, Japan

关键词: Chinese medicine;    collagen fiber;    Makomo;    skin structure;    Zip13-KO mouse;   

DOI  :  10.1292/jvms.17-0206

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Ehlers-Danlos syndrome (EDS) is a group of disorders caused by abnormalities in the extracellular matrix (ECM). Transforming growth factor-β (TGF-β) plays a crucial role in formation of the ECM by the SMAD (Sma-and Mad-related protein, mothers against decapentaplegic homolog) pathway. It has been reported that loss of function of zinc transporter ZRT/IRT-like protein 13 (ZIP13) is the cause of the spondylocheiro dysplastic form of EDS (SCD-EDS OMIM 612350). Our previous study suggested that TGF-β1 has a relationship with the skin pathological condition in the Zip13-Knockout (KO) mouse, which is a model of SCD-EDS. Thus far, effective treatment based on modern medicine for this syndrome has not yet been established. According to an approach of traditional Chinese medicine, the present study investigates the medicinal effects of Makomo (Zizania latifolia) on certain aspects of SCD-EDS, such as skin morphology and plasma TGF-β1, in Zip13-KO mice. Increases in densities of collagen fibers and fibrils without a significant change in thickness of the dermal layer were observed in the group of mice fed a Makomo-containing diet. No change in the amount of collagen suggests that Makomo feed does not elevate collagen synthesis, but changes the length of glycosaminoglycan chains and decreases the distance between collagen fibrils. In conclusion, the changes of the skin structure suggest that Makomo can increase the mechanical strength of skin.

【 授权许可】

Unknown