Immunohistochemical localization of inhibin/activin subunits in adult Asian elephant (Elephas maximus) testes

The Journal of Veterinary Medical Science

Sirinart CHAICHANATHONG¹  Kazuyoshi TAYA²  Apichaya SUDSUKH³  Gen WATANABE⁴  Worawidh WAJJWALKU⁵  Nikorn THONGTIP⁵  Kentaro NAGAOKA⁵ 

[1] Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand;Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand;Cooperative Division of Veterinary Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan;Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen campus, Nakhon Pathom 73140, Thailand;Laboratory of Veterinary Physiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan

关键词: Asian elephant;    immunohistochemistry;    inhibin/activin subunits;    Leydig cell;    Sertoli cell;   

DOI  :  10.1292/jvms.17-0531

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Immunolocalization of inhibin-α and inhibin/activin βA and βB subunits in the testes of Asian elephant was determined. Testicular sections were immunostained with polyclonal antisera against inhibin subunit-α and inhibin/activin βA and βB using the avidin-biotin-peroxidase complex method. Positive immunostaining against inhibin-α subunit was strongly present in Sertoli cells, and positive immunostaining for the inhibin/activin βA and βB subunits was observed in both Sertoli and Leydig cells. These results indicated that while Sertoli cells are the predominant source of inhibin and activin secretions in the testes of adult male Asian elephant, Leydig cells are a source of activin but not inhibin.

【 授权许可】

Unknown