Induced pluripotent stem cells-derived myeloid-derived suppressor cells regulate the CD8+ T cell response - ScienceDirect

Stem Cell Research

Daniel Joyce¹ 

关键词: Myeloid-derived suppressor cells;    Induced pluripotent stem cells;    T cell response;   

DOI  :  10.1016/j.scr.2018.03.009

学科分类：生物科学（综合）

来源: Elsevier

【 摘 要 】

Myeloid-derived suppressor cells (MDSCs) are markedly increased in cancer patients and tumor-bearing mice and promote tumor growth and survival by inhibiting host innate and adaptive immunity. In this study, we generated and characterized MDSCs from murine-induced pluripotent stem cells (iPSCs). The iPSCs were co-cultured with OP9 cells, stimulated with GM-CSF, and became morphologically heterologous under co-culturing with hepatic stellate cells. Allogeneic and OVA-specific antigen stimulation demonstrated that iPS-MDSCs have a T-cell regulatory function. Furthermore, a popliteal lymph node assay and autoimmune hepatitis model showed that iPS-MDSCs also regulate immune responsiveness in vivo and have a therapeutic effect against hepatitis. Taken together, our results demonstrated a method of generating functional MDSCs from iPSCs and highlighted the potential of iPS-MDSCs as a key cell therapy resource for transplantation and autoimmune diseases.

【 授权许可】

Unknown